不同温度短期暴露对斑马鱼雄性焦虑及恐惧反应的影响
DOI:
作者:
作者单位:

重庆师范大学

作者简介:

通讯作者:

基金项目:


Effects of short-term exposure at different temperatures on anxiety and fear responses of male zebrafish
Author:
Affiliation:

Chongqing Normal University

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    【目的】探究不同温度短期暴露对斑马鱼(Danio rerio)焦虑及恐惧反应的影响。【方法】分别设置空白组(27℃)、对照组(27℃)、低温组(20℃)和高温组(34℃)。将斑马鱼雄性暴露于相应温度7 d后,对各组测试鱼进行新缸潜游试验。焦虑行为测试结束后,对照组、低温组和高温组施以警戒素刺激,并对其恐惧反应进行行为学测试。【结果】与对照组焦虑反应相比,低温组斑马鱼雄性首次上浮时间、底层停留时间及冻结总时长均有明显增长;而高温组斑马鱼雄性首次上浮时间、冻结总时间均显著减少。在恐惧行为测试结果中,AS能显著升高对照组斑马鱼首次上浮时间、底层停留时间及冻结总时长。与对照组相比,低温能显著增加雄性首次上浮时间、底层停留时间及冻结总时长;而高温则能显著降低雄性首次上浮时间、底层停留时间。【结论】低温暴露显著增强斑马鱼雄性的焦虑行为和恐惧行为;而高温暴露后显著抑制斑马鱼雄性的焦虑行为和恐惧行为。

    Abstract:

    [Purposes] Investigate the effects of short-term exposure of different temperatures on anxiety and fear responses of zebrafish. [Methods] Adult zebrafish males were exposed to blank (27℃), control (27 oC), low (L-T, 20 oC) and high temperatures (H-T, 34 oC) for 7 days. After exposure, novel tank diving test was conducted in each group. After anxiety response test, male fish in blank was stimulated with distilled water, while fish in rest groups were further stimulated with alarm substances (AS). Fear responses were then analyzed thereafter by behavioral tests. [Findings] Compared with anxiety control (27 ℃), the onset time to higher half, duration in lower half and total duration of freezing of male zebrafish in L-T group (20℃) were significantly increased. In contrast, the onset time to higher half and total duration of freezing of zebrafish males in H-T (34℃) group were significantly reduced. For fear response test, AS significantly increased the onset time to higher half, duration in lower half and total duration of freezing of zebrafish in control group. Compared with control group, L-T significantly increased the onset time to higher half, duration in lower half and total duration of freezing of zebrafish males, wihle H-T significantly reduced the onset time to higher half and duration in lower half of zebrafish males. [Conclusions] Results showed that anxiety and fear responses of male zebrafish were significantly enhanced after low temperature exposure, while decreased significantly after high temperature exposure.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2023-09-10
  • 最后修改日期:2023-12-18
  • 录用日期:2024-02-26
  • 在线发布日期: