徐长卿根提取物对类风湿性关节炎的治疗作用及分子机制研究
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重庆师范大学 生命科学学院 教育部活性物质生物技术工程研究中心 重庆市生物活性物质工程研究中心

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重庆森林改革发展基金,国家自然科学基金


Inhibitory effect and molecular mechanism of Cynanchum paniculatum extract on rheumatoid arthritis
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Chongqing Engineering Research Center of Bioactive Substance,Ministry of Education Engineering Research Center of Active Substance and Biotechnology,College of Life Sciences,Chongqing Normal University

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Chongqing Forestry Reform and Development Fund,National Natural Science Foundation of China

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    摘要:

    【目的】探究徐长卿(Cynanchum paniculatum)根提取物对类风湿性关节炎(RA)的治疗作用以及揭示其潜在的分子机制。【方法】采用完全弗氏佐剂(CFA)构建抗原诱导性类风湿性关节炎(AIA)大鼠模型,并分别口服给予不同浓度的徐长卿根提取物。随后,对大鼠的足跖肿胀、体质量、器官指数进行了测量,同时利用ELISA检测血清中促炎因子的水平,测定脚踝组织中髓过氧化物酶(MPO)含量,并通过RT-qPCR检测核因子-κB(NF-κB)通路关键基因的mRNA表达水平。此外,采用GC-MS对徐长卿根提取物的成分进行了分析鉴定。【结果】与模型组相比,徐长卿根提取物实验组能有效消除大鼠的足趾肿胀,且血清中的肿瘤坏死因子-α(TNF-α)和白介素6(IL-6)水平以及脚踝组织中的MPO含量明显降低(p<0.001),并且呈现剂量依赖性。此外,大鼠的体质量和免疫器官的生长发育并没有受到徐长卿根提取物的影响。同时,RT-qPCR结果显示,与模型组相比,甲氨蝶呤组和实验组500 mg?kg-1、250 mg?kg-1组徐长卿根提取物显著降低NF-κB、TNF-α、IL-6和白介素1β(IL-1β)基因的mRNA表达水平(p<0.001),且呈现剂量依赖性。GC-MS结果显示,徐长卿根提取物主要含有丹皮酚、棕榈酸、亚油酸、5-羟甲基糠醛等物质。【结论】徐长卿根提取物表现出良好的RA治疗效果,可能主要通过丹皮酚和亚油酸发挥作用。其治疗分子机制可能涉及调节NF-κB通路。

    Abstract:

    Abstract: [Purposes]To investigate the therapeutic effects of Cynanchum paniculatum root extract on rheumatoid arthritis (RA) and to reveal the underlying molecular mechanisms. [Methods]A rat model of antigen-induced rheumatoid arthritis (AIA) was constructed using complete freund''s adjuvant (CFA), and different concentrations of C. paniculatum root extract were administered orally. Subsequently, paw swelling, body weight, and organ index of rats were measured, and ELISA was used to detect serum pro-inflammatory factor levels, myeloperoxidase (MPO) content in ankle tissues, and RT-qPCR to detect the mRNA expression levels of key genes of the NF-κB pathway.In addition, GC-MS was used to analyze and identify the components of the C. paniculatu root extract. [Findings] Compared with the model group, the experimental group of C. paniculatu root extract effectively eliminated paw swelling in rats.And serum levels of tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) as well as MPO content in ankle tissues were significantly reduced (p<0.001) in a dose-dependent manner. In addition, the body weight and the growth and development of immune organs of rats were not affected by the extract of C. paniculatum root. Meanwhile, RT-qPCR results showed that the mRNA expression levels of NF-κB, TNF-α, IL-6, and interleukin 1β (IL-1β) genes were significantly (p<0.001) decreased by the methotrexate group and the experimental group with 500 mg?kg-1 and 250 mg?kg-1 of C. paniculatum root extract compared with the model group in a dose-dependent manner..GC-MS results showed that the root extract of C. paniculatu mainly contained paeonol, palmitic acid, linoleic acid, and 5-hydroxymethylfurfural.[Conclusions]C. paniculatum root extract showed good RA therapeutic effects, which may act primarily through paeonol and linoleic acid, and the preliminary reveals that its therapeutic mechanism may be related to the regulation of NF-κB pathway.

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  • 收稿日期:2023-09-27
  • 最后修改日期:2024-03-18
  • 录用日期:2024-04-08
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