Abstract:[Objective] To examine the effect of metabolic range on spatial location and group behavior of Chinese barb (Spinibarbus sinensis). [Methods] The metabolic rate was measured at (25.4 ± 0.2) °C and the metabolic range was calculated. [Results] Oxygen consumption rate increased significantly with increasing food intake, and its residual metabolic range was predicted to be significantly negatively correlated with food intake (p<0.001). The peak feeding metabolism showed an overall upward trend. The higher the fed individual, the more forward the average spatial position of the shoal (p<0.001). There was no significant difference in the mean spatial position of individuals in shoal at different times after feeding, but the low AS group was significantly higher than that of individuals with high AS in the group 1 position (p<0.001). After feeding, the inter-individual distance increased significantly and the group polarity decreased significantly (p<0.05). [Conclusion] Metabolic range had significant effect on the spatial position of Chinese barb, and individuals with low AS occupied the leading position of the group for a longer period of time. After feeding, the cohesion of shoal is decreased, as did group coordination.