Clarifying the balance between supply and demand of ecosystem services and scientifically carrying out ecological spatial zoning are important prerequisites for the implementation of differentiated ecological management. Take the Chongqing Three Gorges Reservoir area as an example, based on multi-source data, and takes the township as the research unit to quantitatively assess the relationship between the total supply and demand ratio of regional ecosystem services and the coordination degree, constructed an ecological spatial zoning method based on the balance of supply and demand of ecosystem services, delimit ecological zoning, and proposed targeted optimization management strategies. The results show that: from 2000 to 2020, 1) ecosystem services in the Chongqing Three Gorges Reservoir area are dominated by surpluses, with the most mild surpluses and deficits located mainly in the central urban areas; the areas where the total supply/demand ratio is trending toward increasing and decreasing are roughly equal, with growth dominating the townships in the north-central and southwestern parts of the study area. 2) The coordination degree of ecosystem services showed the spatial distribution characteristics of "high in northeast and low in southwest". The change in coordination degree is dominated by a decrease, which is mainly distributed in the northeastern part of the study area, and the growth area is concentrated in the southern part of the study area. 3) Coupled with the change of ecosystem services supply and demand ratio and the change of coordination degree, the Chongqing Three Gorges Reservoir area was divided into four types: key ecological restoration area, ecological restoration area, ecological potential restoration area and ecological core protection area. This paper proposes differentiated optimization strategies for corresponding ecological zones to provide scientific support for land resource regulation and ecological restoration.