Abstract:[Purposes] In order to realize the high-speed and high-resolution imaging of the biological tissue tomography, a set of optical coherence tomography imaging system is developed based on the swept. [Methods] The hardware of the system is based on the 1 310 nm center wavelength frequency Scanning laser source, and the software system compiled by LabVIEW is used as the processing tool to carry out experiments on the animal and plant epidermis. [Findings] The results show that the system can realize the high-speed acquisition and processing of 1 million data points within 5 s, achieve the high-resolution optical imaging of 10 μm, and the imaging depth can reach 2.3 mm, which can fully meet the imaging needs of biological tissue tomography. [Conclusions] In addition, the system also has the characteristics of small volume, simple operation and strong portability, which is enough to meet the optical imaging requirements of clinical biomedical scenes.