Abstract:[Purposes]It measured the visceral organs index of Rana catesbeiana and analyzed the relationship between various quantitative characteristics, and obtained breeding indicators for R. catesbeiana. [Methods]After dissecting 42 female and 38 male bullfrogs and obtaining the quantifiable data of body length, body width, head length, body mass and organ mass, the relationship between those different quantifiable characteristics of R. catesbeiana were analyzed based on several statistical methods such as independent sample t-test, Mann-Whitney U-test and multiple regression analysis. [Results]All the correlation coefficients between each pair of quantifiable characteristics were significant (p<0.01). The head-to-body ratio and bilateral lung index of the males were larger than that of the females, and the differences were significant (p<0.01). The gonad index of the females was larger than that of the males, and the difference was significant (p<0.01); while there was no significant difference in liver index, heart index, kidney index and fat body index between the males and the females. The main quantifiable characteristics most closely related to the body mass for females were body width, head length, fat body mass, gonad mass and bilateral lung mass, however, the main quantifiable characteristics most closely related to the body mass for males were body width, body length, fat body mass, heart mass and bilateral lung mass. [Conclusions]According to the above series of studies, it was conclude that there are many factors that affect the body mass of the females and the males. Except those common factors (such as body width and fat body mass), some different ones (such as head length, gonad mass and heart mass) were found for those female and male individuals, which indicated that there were certain sexual differences existed in the main characterization components that affect body mass. Based on the above studies, it was conclude that body mass, body length, body width and head length could be used as breeding indicators in the breeding of R. catesbeiana, among which body mass was the preferred breeding indicator, and body width was the secondary breeding indicator.