To investigate the effects of the interaction of arbuscular mycorrhizal networks and calcium carbonate on the nitrogen and phosphorus nutrients of Cirtnamomum camphora seedlings in non-rhizosphere soil. The plant rhizosphere and non-rhizosphere compartment devices were simulated and constructed, the nylon meshes isolation was used to realize the interconnection of mycorrhizal networks between rhizosphere and non-rhizosphere compartments, the rhizosphere compartment was planted with Cirtnamomum camphora seedlings with arbuscular mycorrhizal fungi, the non-rhizosphere compartment was applied to add or not add calcium carbonate. After the cultivation period, the biomass, the nutrient content of nitrogen and phosphorus of the plant were determined. The results showed that mycorrhizal networks had a significant impact on the biomass, the nitrogen and phosphorus acquisition, and the nitrogen/phosphorus ratio of camphor tree seedlings; the application of calcium carbonate had a significant impact on the nitrogen content, nitrogen acquisition, and nitrogen/phosphorus ratio of seedling plants; after adding calcium carbonate, the mycorrhizal networks significantly increased the biomass, nitrogen content, nitrogen and phosphorus acquisition of seedling plants, the nitrogen and phosphorus acquisition of leaves, and the nitrogen/phosphorus ratio of roots; applying calcium carbonate in the presence of mycorrhizal networks can significantly increase the nitrogen content of seedling plants, the nitrogen acquisition of roots and leaves, and nitrogen/phosphorus ratio of roots. The interaction of mycorrhizal networks and calcium carbonate significantly affected the nitrogen content, the nitrogen and phosphorus acquisition, the nitrogen/phosphorus ratio of seedlings but did not for the biomass of seedling plants. The results suggest that the interaction of arbuscular mycorrhizal networks and calcium carbonate can promote the absorption and utilization of nitrogen and phosphorus by plants in karst non-rhizosphere soil.