The aim of the present study was to investigate the effects of temperature and feeding on the energy metabolism trait and hypoxia tolerance of fish. The routine metabolic rate (RMR), critical oxygen pressure (COP), and loss of equilibrium (LOE) of the control group (fasting group) and the feeding group of southern catfish were measured at 15, 20, 25, and 30 ℃, respectively. Temperature has a significant impact on RMR, COP, and LOE, and these parameters significantly increase with increasing temperature (p<0.05). Feeding has a significant impact on RMR and COP. Indicated that increasing temperature would weaken the hypoxia tolerance of southern catfish, and feeding would weaken the hypoxia tolerance of this species at low temperature. As well as, feeding had some compensatory effect on hypoxia tolerance at high temperature in this species, and therefore might be related to factors such as energy metabolism characteristic, physiological regulation capability and body acid-base balance at different temperatures.