It was explored whether ethanol extracts from different parts of four species of Asteraceae invasive plants such as Eupatorium adenophora, Eupatorium odoratum, Bidens pilosa, and Galinsoga parviflora had antifungal activity against five strains of plant pathogens such as Alternaria brassicae, Botrytis cinerea, Phytophthora cactorum, Fusarium sp., and Fusarium chlamydosporum, in order to provide theoretical foundation for further development of new botanical pesticides and comprehensive management of Asteraceae invasive plants. Ethanol extracts from different parts of four invasive Asteraceae plants were prepared by ethanol reflux extraction, and their antifungal activities against five plant pathogens were determined by filter paper inhibition zone method and mycelial growth rate method. The results showed that the ethanol extracts of four invasive plants at the mass concentration of 5 mg·mL-1 showed selective inhibitory effects on five pathogens, and among them the flower ethanol extract of E. adenophora showed the best inhibitory effect on the growth of A. brassicae mycelium, with EC50 of 0.086 8 mg·mL-1; the stem and leave ethanol extract of E. adenophora and the aerial part ethanol extract of B. pilosa display potent inhibition effects on the mycelial growth of F. chlamydosporum and their EC50 were 0.091 3 and 0.194 3 mg·mL-1, respectively. The results suggested that the ethanol extracts of E. adenophora and B. pilosa showed significant antifungal activity, especially the aerial part ethanol extract of E. adenophora worth further study.