In order to investigate the effects and relationship of feeding frequency on growth, digestive capacity and ingestion metabolism of largemouth bass (Micropterus salmoides). The specific growth rate (SGR), feeding rate (FR), feed efficiency (FE), changes in stomach contents and special dynamic action (SDA) were measured after feeding at different frequencies (1 time per day, 2 times per day and 3 times per day) at 25 ℃. The results showed that the end body weight and body length of fish in each group increased significantly after feeding for 28 days. With the increase of feeding frequency (2 times per day and 3 times per day), SGR increased by 11%~14%, FR increased by 16%~18%, and FE did not change significantly. There was a significant positive correlation between SGR and FR (p<0.05). The results showed that the growth ability of largemouth bass was enhanced by increasing feeding frequency mainly due to the increase of daily feed intake. With the increase of feeding frequency (2 times per day and 3 times per day), the daily food intake increased by 16%~18%, and the emptying time only increased by 2~4 h. The results indicated that increasing the feeding frequency of largemouth bass had a superposition effect on gastric contents emptying. The results showed that there were no significant differences in resting metabolic rate (RMR), peak feeding metabolic rate (PMR) and peak feeding metabolic scope (PMS) among different feeding frequency groups. With the increase of feeding frequency, PMR and PMS increased by about 10% respectively (the increase was not significant). The SDA duration increased by about 43%, the total SDA energy consumption increased by more than 70%, and the SDA coefficient increased by about 45% (p<0.05). The results indicated that the assimilation ability of largemouth bass was enhanced with the increase of daily food intake. Therefore, based on the growth performance of largemouth bass, it is appropriate to feed twice a day during the breeding process, with a feeding interval of about 12 h. These are related to the daily intake, digestive capacity and metabolic reaction of food intake.