To investigate whether zebrafish (Danio rerio) have a visual preference for adult heterosexual conspecifics with or without breeding experience, adult heterosexual zebrafish with and without breeding experience were placed as stimulus fish on both sides of the tank containing male (or female) subject fish during the zebrafish breeding season, and after taking videos of the subjects’ preference behaviors for 10 min, their parameters related to the preference behavior test were analyzed. The results showed that: 1) The duration and frequency of hitting the wall of the preference zone with breeding experience, the total duration and frequency of hitting the wall of both preference zones, the frequency of closing to each preference zone, and the total frequency of closing to each preference zone, were all significantly higher in male zebrafish than in female test fish. 2) The duration and frequency of hitting the wall of the preference zone with breeding experience was significantly more than that of hitting the wall of the preference zone with no breeding experience, the duration of male zebrafish closing the breeding experience preference area was significantly more than that of closing the non-breeding experience preference area. These results suggest that male zebrafish dominate the breeding process and have a stronger visual preference for female zebrafish with breeding experience, while female zebrafish are in a subordinate position during the breeding process and do not have a clear preference for mates with or without breeding experience.