To explore the sex preference of zebrafish (Danio rerio) females to their mates during reproduction, the control (visual and olfactory signals stimuli), olfactory (olfactory signals stimuli) and optical (visual signals stimuli) groups were set. During reproduction (8:00—10:00), female fish were placed in the onset zone individually, and stimulus fish were placed in the male and female stimulation zone respectively. The sexual preference of zebrafish was determined for 10 minutes after light stimuli. The results showed that: 1) No significant difference of the frequency in the zone was found in females of each group. 2) In the control and optical groups, frequency of striking, duration in the zone and maximum of duration of the female were all significantly higher when stimulated by the males. 3) In olfactory group, no significant difference of the frequency of striking, duration in the zone and maximum of duration in the female was found between sexes. The results indicated that zebrafish female significantly prefered males during reproduction and likely through an optical-triggered pathway.