Fifteen juvenile of Macropodus opercularis of uniform size and optimal health were utilized as experimental subjects to investigate their spatial learning capabilities. The experiment was conducted in a cross-shaped maze with road signs as visual cues, where each experimental fish underwent continuous training for 15 cycles, with one training session at noon and one at night each day, totaling 30 training sessions. To determine the spatial learning ability of M. opercularis by comparing the differences in learning outcomes and movement characteristics before and after spatial learning training, and to explore the changes in its locomotor characteristics during this process.Results indicated a significantly positive correlation between the number of training sessions and percentage correct of M. opercularis (p<0.05), while foraging time and the number of reversal exhibited significantly negative correlations with the number of training sessions (p<0.05). The individual swimming speed, individual swimming acceleration, percent time spent on moving， and other motion characteristic parameters of M. opercularis did not change significantly before and after training. The research results indicate that M. opercularis possesses a certain capacity for spatial learning, can establish the connection between food and visual landmarks, and there are no changes significantly in locomotor characteristics during the learning process.