The long-standing stagnation in mammal cataloging for Chongqing underscores the urgency of systematic updates to its species records. Through integrating published literature and contemporary field survey data, a systematic reorganization of Chongqing’s mammalian fauna was conducted using Survey on Resources and Fauna of Mammals in Chongqing City as a foundational dataset. Taxonomic adjustments align with Taxonomy and Distribution of Mammals in China and Catalogue of Mammals in China (2024 edition), resolving historical ambiguities in species classification and geographic distribution boundaries. Comparative analyses of species diversity, faunal composition, and spatial distribution patterns reveal updated insights into Chongqing’s mammalian biodiversity framework.As of late December 2024, Chongqing records 132 mammal species belonging to 8 orders, 30 families, and 85 genera. Compared to previous records, 28 species were newly added, 12 species were removed, 7 species were listed with doubts, and 3 species were not recorded. Conservation status assessments reveal 10 Class I and 14 Class II nationally protected mammals, include 24 species listed as threatened mammals in the China Biodiversity Red List, and identify 28 species endemic to China. Taxonomic revisions for selected species included reclassification and nomenclatural updates. Biogeographically, the fauna is dominated by 76 Oriental realm species, followed by 54 widespread species and 2 Palearctic realm species. Spatial analysis of the G-F diversity index (geographic distribution-faunal richness index) identifies Pendong Mountainous and Hilly Area, Daba Mountain range, Three Gorges Valley, and Pendong Parallel Ridge Valley as high-diversity hotspots, while Dalou Medium Mountain Valley, Wuling Mountain range, and Sichuan Basin area exhibit lower richness. These updated data provide a taxonomically validated framework for biodiversity monitoring, habitat prioritization, and evidence-based conservation planning in Chongqing.