Clarifying the balance between supply and demand of ecosystem services and scientifically carrying out ecological spatial zoning are important prerequisites for the implementation of differentiated ecological management. Taking the Three Gorges Reservoir Area of Chongqing as the study area, based on multi-source data and township-level townships as the research unit, it quantitatively evaluates the change relationship between the total supply-demand ratio and coordination degree of ecosystem services in the study area from 2000 to 2020, constructs an ecological spatial zoning method based on the balance of supply and demand of ecosystem services, delineates the ecological zoning and proposes a targeted optimization management strategy.The results show that: 1) Ecosystem services in the study area are dominated by surpluses, with the largest slight surplus, while the deficits located mainly in the central urban areas; the areas where the total supply/demand ratio is mainly decreasing, with growth dominating the townships in the north-central and southwestern parts of the study area. 2) The coordination degree of ecosystem services showed the spatial distribution characteristics of “high in northeast and low in southwest”. The change in coordination degree is dominated by a increase, which is mainly distributed in the northeastern part of the study area, while the areas with decreased coordination degree were concentrated in the northeast of the study area. 3) Coupled with the change of ecosystem services supply and demand ratio and the change of coordination degree, the study area was divided into four types: key ecological restoration area, ecological restoration area, ecological potential restoration area and ecological core protection area. It proposes differentiated optimization strategies for corresponding ecological zones to provide scientific support for land resource regulation and ecological restoration.