To investigate whether fear contagion in zebrafish (Danio rerio) exhibits sexual dimorphism, both male and female zebrafish used in the experiment were divided into control males, stimulated males, control females, and stimulated females. Subsequently, fear contagion tests between same/opposite-sex individuals were conducted on these groups. Distilled water and alarm substance were administered to demonstrator fish in the control group and stimulus group, respectively. Behavioral data related to fear responses were recorded and analyzed. Results from the fear contagion tests between same-sex individuals showed that, the latency to enter upper layer and total duration spent in lower layer of stimulated males/females were significantly longer than those of control males/females; there were no significant differences in rise frequency and dive frequency between control and stimulated males; the rise frequency and dive frequency of stimulated females were significantly lower than those of control females. Results from the fear contagion tests between opposite-sex individuals showed that, there were no significant differences in all behavioral indicators between control and stimulated males; the latency to enter upper layer and total duration spent in lower layer of stimulated female were significantly longer than those of control female, while rise frequency and dive frequency showed no significant changes between control and stimulated females. The study results indicate that fear contagion in zebrafish exhibits sexual dimorphism, with female zebrafish displaying fear contagion between same/opposite-sex individuals, whereas male zebrafish exhibit fear contagion only among same-sex individuals.