Animal personality refers to consistent individual differences in behavior among animals, which may have potential impacts on survival, reproduction, and population interactions. This study employed standardized behavioral tests and multivariate statistical analyses to investigate the behavioral syndrome of male Macropodus opercularis , particularly the interrelationships among boldness, activity, and aggressiveness. Results show that male M . opercularis exhibits high individual variation in personality traits within the species, and the three traits are significantly positively correlated—individuals with higher aggressiveness displays greater activity and boldness when exposed to novel stimuli. Based on these findings, this study further discusses the correlations among personality traits in male M . opercularis and their ecological and evolutionary implications. The results provide insights into understanding behavioral syndromes in non-model species and offer new perspectives on the adaptive significance of individual personality differences and behavioral diversity in natural populations.