Although sulfadiguanide has a broad-spectrum antibacterial effect as a drug used in clinical practice, excessive intake is fatal to the human body, so in order to determine the content of sulfadiguanide in drugs and reduce the cost of detection, a new chemiluminescence method for the determination of sulfadiguanide content by combined flow injection technology was established. Under the acid condition, the sulfadiguanide is determined by potassium permanganate-formaldehyde luminescent system combined with flow injection chemiluminescence analyzer. The result show that the linear range is 5×10 -5 ～6×10 -4 mol·L -1 , the detection limit is 6×10 -7 mol·L -1 , the relative standard deviation is 1.1% ( n =11, C sulfadiguanide =2×10 -4 mol·L -1 ), the recovery is 97%～ 103%. Using this method has been successful to the detection of sulfadiguanide recognition with satisfactory result.